SM4816 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification unlocked nucleic acid unlocked nucleic acid
siRNA modification types 1 1
Overall number of modifications 2 3
Position of modifications 20,21 2,20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCCOC(CO)CO



OCCOC(CO)CO



siRNA Sequence GGACAUUCAGAACAAGAAAUU UUUCUUGUUCUGAAUGUCCUU
siRNA length base-pair 21 21
siRNA name in paper UNAG1 UNAG1
Biological activity 36 percent target mRNA inhibition
Experiment used to check activity qRT PCR
Melting temperature (oC) NA
Target gene ApoB gene
siRNA concentration 5nM
Cell or Organism used HepG2 cells
Transfection method RNAiMAX
Duration after transfection 24 Hoursrs
Article title Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs
Reference21047800