| Field | Sense | Antisense |
| siRNA chemical modification | 2,4-bridged nucleic acid* Phosphorothioate* DeoxyThymidine* Cholesterol | 2-Methoxyribose* DeoxyThymidine |
| siRNA modification types | 4 | 2 |
| Overall number of modifications | 18 | 4 |
| Position of modifications | 1,2,5,15,18 * 3,4,6,7,16,17,19,20,21,22 * 23,24 * 24 | 13,15 * 23,24 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O OCC1OCC(O)C1OP(O)(S)=O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C |
COC1C(O)OC(CO)C1O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | GTCATCACACUGAATACCAAUUTT | AAUUGGUAUUCAGUGUGAUGACTT |
| siRNA length base-pair | 24 | 24 |
| siRNA name in paper | siL2PTC-1M | siL2PTC-1M |
| Biological activity | 27 percent target mRNA inhibition | |
| Experiment used to check activity | Quantitative PCR analysis | |
| Melting temperature (oC) | 84.9 | |
| Target gene | apoB gene | |
| siRNA concentration | 10nM/L | |
| Cell or Organism used | NMuLi cells | |
| Transfection method | Lipofectamine RNAiMAX | |
| Duration after transfection | NA | |
| Article title | Development of a 2',4'-BNA/LNA-based siRNA for Dyslipidemia and Assessment of the Effects of Its Chemical Modifications In Vivo |
|
| Reference | 23344237 | |