Field | Sense | Antisense |
siRNA chemical modification | DeoxyThymidine | DeoxyThymidine |
siRNA modification types | 1 | 1 |
Overall number of modifications | 2 | 2 |
Position of modifications | 20,21 | 20,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
siRNA Sequence | GUAUCUCUUCAUAGCCUUATT | UAAGGCUAUGAAGAGAUACTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | siNTCtrl | siNTCtrl |
Biological activity | NA | |
Experiment used to check activity | Quantitative PCR analysis | |
Melting temperature (oC) | N.D. | |
Target gene | apoB gene | |
siRNA concentration | 10nM/L | |
Cell or Organism used | NMuLi cells | |
Transfection method | Lipofectamine RNAiMAX | |
Duration after transfection | NA | |
Article title | Development of a 2',4'-BNA/LNA-based siRNA for Dyslipidemia and Assessment of the Effects of Its Chemical Modifications In Vivo |
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Reference | 23344237 |