Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-O-Methyl |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 6 |
Position of modifications | 0 | 14,15,16,17,18,19 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
COC1C(O)OC(CO)C1O |
siRNA Sequence | CGUACGCGGAAUACUUCGATT | UCGAAGUAUUCCGCGUACGTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | RNA21 | RNA21 |
Biological activity | 30.8 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase assay | |
Melting temperature (oC) | NA | |
Target gene | Photinus luciferase gene | |
siRNA concentration | 25 nM | |
Cell or Organism used | NIH/3T3 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 24 Hours | |
Article title | RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells |
|
Reference | 15919084 |