SM4911 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 4-Thioribose
siRNA modification types 0 1
Overall number of modifications 0 4
Position of modifications 0 1,2,18,19
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1SCC(O)C1O



siRNA Sequence CGUACGCGGAAUACUUCGATT UCGAAGUAUUCCGCGUACGTT
siRNA length base-pair 21 21
siRNA name in paper RNA19 RNA19
Biological activity 88.8 percent target mRNA inhibition
Experiment used to check activity Dual luciferase assay
Melting temperature (oC) NA
Target gene Photinus luciferase gene
siRNA concentration 25 nM
Cell or Organism used NIH/3T3 cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Study of modification pattern-RNAi activity relationships by using siRNAs modified with 4'-thioribonucleosides
Reference17924376