| Field | Sense | Antisense |
| siRNA chemical modification | 4-Thioribose | 4-Thioribose |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 8 | 4 |
| Position of modifications | 1,2,3,4,16,17,18,19 | 16,17,18,19 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1SCC(O)C1O |
OCC1SCC(O)C1O |
| siRNA Sequence | CGUACGCGGAAUACUUCGATT | UCGAAGUAUUCCGCGUACGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | RNA33 | RNA33 |
| Biological activity | 88.1 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase assay | |
| Melting temperature (oC) | NA | |
| Target gene | Photinus luciferase gene | |
| siRNA concentration | 25 nM | |
| Cell or Organism used | NIH/3T3 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Study of modification pattern-RNAi activity relationships by using siRNAs modified with 4'-thioribonucleosides |
|
| Reference | 17924376 | |