| Field | Sense | Antisense |
| siRNA chemical modification | Inverted abasic* 2-Deoxy* 2-Fluoro | 2-Fluoro* 2-O-Methyl |
| siRNA modification types | 3 | 2 |
| Overall number of modifications | 23 | 21 |
| Position of modifications | 1,21 * 1,3,4,5,6,9,11,12,13,14,15,16,17,19,20,21 * 2,7,8,10,18 | 4,5,6,7,8,9,11,14,15,16,17,19 * 10,12,13,18,20,21 |
| Modification on sugar or base or phosphate | Base; Sugar; Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1COC(CO)C1OP(O)([O-])=O OCC1OCCC1O OCC1OCC(F)C1O |
OCC1OCC(F)C1O COC1C(O)OC(CO)C1O |
| siRNA Sequence | AUAAGGCUAUGAAGAGAUATT | UAUCUCUUCAUAGCCUUAUUU |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | Luc(80) | Luc(80) |
| Biological activity | High target mRNA inhibition efficacy | |
| Experiment used to check activity | Real-time qPCR | |
| Melting temperature (oC) | NA | |
| Target gene | Luciferase gene | |
| siRNA concentration | ||
| Cell or Organism used | Transgenic mice | |
| Transfection method | Lipid nanoparticles | |
| Duration after transfection | NA | |
| Article title | RNA-induced silencing complex-bound small interfering RNA is a determinant of RNA interference-mediated gene silencing in mice |
|
| Reference | 21427169 | |