SM4956 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Inverted abasic* 2-Deoxy* 2-Fluoro 2-Fluoro* 2-O-Methyl
siRNA modification types 3 2
Overall number of modifications 23 21
Position of modifications 1,21 * 1,3,4,6,7,8,13,14,16,18,19,20,21 * 2,5,9,10,11,12,15,17 4,6,7,12,13,14,16,17,19 * 5,8,9,10,11,15,18,20,21
Modification on sugar or base or phosphate Base; Sugar; Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC1COC(CO)C1OP(O)([O-])=O
OCC1OCCC1O
OCC1OCC(F)C1O

OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O


siRNA Sequence ACAACAGACUUUAAUGUAATT UUACAUUAAAGUCUGUUGUUU
siRNA length base-pair 21 21
siRNA name in paper Ssb(291) Ssb(291)
Biological activity NA
Experiment used to check activity Real-time qPCR
Melting temperature (oC) NA
Target gene Ssb gene
siRNA concentration
Cell or Organism used Transgenic mice
Transfection method Lipid nanoparticles
Duration after transfection NA
Article title RNA-induced silencing complex-bound small interfering RNA is a determinant of RNA interference-mediated gene silencing in mice
Reference21427169