SM4957 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Inverted abasic* 2-Deoxy* 2-Fluoro 2-Fluoro* 2-O-Methyl
siRNA modification types 3 2
Overall number of modifications 23 21
Position of modifications 1,21 * 1,3,5,6,9,10,13,15,17,19,20,21 * 2,4,7,8,11,12,14,16,18, 5,7,10,11,14,15,17,19 * 4,6,8,9,12,13,16,18,20,21
Modification on sugar or base or phosphate Base; Sugar; Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC1COC(CO)C1OP(O)([O-])=O
OCC1OCCC1O
OCC1OCC(F)C1O

OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O


siRNA Sequence GUAUGACCGACUACGCGUATT UACGCGUAGUCGGUCAUACUU
siRNA length base-pair 21 21
siRNA name in paper UC3 UC3
Biological activity NA
Experiment used to check activity Real-time qPCR
Melting temperature (oC) NA
Target gene UC3 gene
siRNA concentration
Cell or Organism used Transgenic mice
Transfection method Lipid nanoparticles
Duration after transfection NA
Article title RNA-induced silencing complex-bound small interfering RNA is a determinant of RNA interference-mediated gene silencing in mice
Reference21427169