SM4980 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Inverted abasic* 2-Deoxy* 2-Methoxy 5-phosphate ribose* Inverted abasic* 2-Fluoro* 2-Methoxy
siRNA modification types 3 4
Overall number of modifications 23 20
Position of modifications 1,21 * 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19 * 20,21 1 * 1 * 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16 * 17,18
Modification on sugar or base or phosphate Base; Sugar; Sugar Phosphate; Base; Sugar; Sugar
SMILES
(Click to view structure & nomenclature)
CC1COC(CO)C1OP(O)([O-])=O
OCC1OCCC1O
COC1C(O)OC(CO)C1O

OC1COC(COP(O)([O-])=O)C1O
CC1COC(CO)C1OP(O)([O-])=O
OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O
siRNA Sequence CCAGUAAGGCUUCUCUUAAUU AGAGAAGCCUUACUGGUU
siRNA length base-pair 21 18
siRNA name in paper APOB8786 APOB8786-122
Biological activity 93.3 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene ApoB gene
siRNA concentration 100 nM
Cell or Organism used Hepa1-6 cells
Transfection method Lipofectamine RNAiMAX
Duration after transfection 24 Hours
Article title mRNA knockdown by single strand RNA is improved by chemical modifications
Reference22253019