| Field | Sense | Antisense |
| siRNA chemical modification | Inverted abasic* 2-Deoxy* 2-Methoxy | 5-phosphate ribose* Inverted abasic* 2-Fluoro* 2-Methoxy |
| siRNA modification types | 3 | 4 |
| Overall number of modifications | 23 | 20 |
| Position of modifications | 1,21 * 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19 * 20,21 | 1 * 1 * 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16 * 17,18 |
| Modification on sugar or base or phosphate | Base; Sugar; Sugar | Phosphate; Base; Sugar; Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1COC(CO)C1OP(O)([O-])=O OCC1OCCC1O COC1C(O)OC(CO)C1O |
OC1COC(COP(O)([O-])=O)C1O CC1COC(CO)C1OP(O)([O-])=O OCC1OCC(F)C1O COC1C(O)OC(CO)C1O |
| siRNA Sequence | CACAAUGCAUUUAGAUCAAUU | AUCUAAAUGCAUUGUGUU |
| siRNA length base-pair | 21 | 18 |
| siRNA name in paper | APOB6981 | APOB6981-122 |
| Biological activity | 94.42 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | ApoB gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | Hepa1-6 cells | |
| Transfection method | Lipofectamine RNAiMAX | |
| Duration after transfection | 24 Hours | |
| Article title | mRNA knockdown by single strand RNA is improved by chemical modifications |
|
| Reference | 22253019 | |