| Field | Sense | Antisense |
| siRNA chemical modification | Inverted abasic* 2-Methoxy | 5-hydroxy ribose* 2-Methoxy |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 4 | 3 |
| Position of modifications | 1,21 * 20,21 | 1 * 20,21 |
| Modification on sugar or base or phosphate | Base; Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1COC(CO)C1OP(O)([O-])=O COC1C(O)OC(CO)C1O |
C1COC(C1O)CO COC1C(O)OC(CO)C1O |
| siRNA Sequence | AAAUCAUGGUGAAAUAAAAUU | UUUUAUUUCACCAUGAUUUUU |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | SSB(869) unmodified siRNA | SSB(869) unmodified siRNA |
| Biological activity | EC-50 = 0.02 nM for target mRNA | |
| Experiment used to check activity | Real-time quantitative PCR | |
| Melting temperature (oC) | NA | |
| Target gene | SSB gene | |
| siRNA concentration | NA | |
| Cell or Organism used | Hepa1-6 cells | |
| Transfection method | Lipofectamine RNAiMax | |
| Duration after transfection | 24 Hours | |
| Article title | In vivo activity and duration of short interfering RNAs containing a synthetic 5'-phosphate |
|
| Reference | 22400991 | |