VIRsiRNAdb

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• VsiRNAid

Total number of Results for Spike are 11
Results from 0 - 25

siRNA sequence "gggcuaccaccuuaugucc" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gggcuaccaccuuaugucc" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1289 " record
Length: 19
GC Content:58 %
Starting position: 3081
Strain of Virus: BJ01
GenBank Acc: AY278488
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gggcuaccaccuuaugucc" siRNA in Human Genome sequences
See AY278488 at Genbank
Pubmed:14988013
Article:Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference.
Authors:Zhang Y, Li T, Fu L, Yu C, Li Y, Xu X, Wang Y, Ning H, Zhang S, Chen W, Babiuk LA, Chang Z.
Journal:FEBS Lett. 2004 Feb 27;560(1-3):141-6.
Entrez:14988013

siRNA sequence "gagcuuugagauugacaaa" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gagcuuugagauugacaaa" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1294 " record
Length: 21
GC Content:33 %
Starting position: 403
GenBank Acc: AY278554
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gagcuuugagauugacaaa" siRNA in Human Genome sequences
See AY278554 at Genbank
Pubmed:15504339
Article:Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells.
Authors:Qin ZL, Zhao P, Zhang XL, Yu JG, Cao MM, Zhao LJ, Luan J, Qi ZT.
Journal:Biochem Biophys Res Commun. 2004 Nov 26;324(4):1186-93.
Entrez:15504339

siRNA sequence "cccuuucuuugcuguuucu" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "cccuuucuuugcuguuucu" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1290 " record
Length: 21
GC Content:38 %
Starting position: 871
GenBank Acc: AY278554
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "cccuuucuuugcuguuucu" siRNA in Human Genome sequences
See AY278554 at Genbank
Pubmed:15504339
Article:Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells.
Authors:Qin ZL, Zhao P, Zhang XL, Yu JG, Cao MM, Zhao LJ, Luan J, Qi ZT.
Journal:Biochem Biophys Res Commun. 2004 Nov 26;324(4):1186-93.
Entrez:15504339

siRNA sequence "gggcuaucaaccuauagau" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gggcuaucaaccuauagau" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1347 " record
Length: 19
GC Content:42 %
Starting position: 22085
Strain of Virus: Urbani
GenBank Acc: AY278741
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gggcuaucaaccuauagau" siRNA in Human Genome sequences
See AY278741 at Genbank
Pubmed:15652970
Article:Inhibition of SARS-CoV replication by siRNA.
Authors:Wu CJ, Huang HW, Liu CY, Hong CF, Chan YL.
Journal:Antiviral Res. 2005 Jan;65(1):45-8.
Entrez:15652970

siRNA sequence "gagacauaucuaaugugcc" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gagacauaucuaaugugcc" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1288 " record
Length: 19
GC Content:42 %
Starting position: 1358
Strain of Virus: BJ01
GenBank Acc: AY278488
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gagacauaucuaaugugcc" siRNA in Human Genome sequences
See AY278488 at Genbank
Pubmed:14988013
Article:Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference.
Authors:Zhang Y, Li T, Fu L, Yu C, Li Y, Xu X, Wang Y, Ning H, Zhang S, Chen W, Babiuk LA, Chang Z.
Journal:FEBS Lett. 2004 Feb 27;560(1-3):141-6.
Entrez:14988013

siRNA sequence "gagacauaucuaaugugcc" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gagacauaucuaaugugcc" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1291 " record
Length: 19
GC Content:42 %
Starting position: 1358
Strain of Virus: BJ01
GenBank Acc: AY278488
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gagacauaucuaaugugcc" siRNA in Human Genome sequences
See AY278488 at Genbank
Pubmed:14988013
Article:Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference.
Authors:Zhang Y, Li T, Fu L, Yu C, Li Y, Xu X, Wang Y, Ning H, Zhang S, Chen W, Babiuk LA, Chang Z.
Journal:FEBS Lett. 2004 Feb 27;560(1-3):141-6.
Entrez:14988013

siRNA sequence "caaggcgauuagucaaauu" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "caaggcgauuagucaaauu" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1344 " record
Length: 19
GC Content:37 %
Starting position: 24221
Strain of Virus: Urbani
GenBank Acc: AY278741
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "caaggcgauuagucaaauu" siRNA in Human Genome sequences
See AY278741 at Genbank
Pubmed:15652970
Article:Inhibition of SARS-CoV replication by siRNA.
Authors:Wu CJ, Huang HW, Liu CY, Hong CF, Chan YL.
Journal:Antiviral Res. 2005 Jan;65(1):45-8.
Entrez:15652970

siRNA sequence "cacugauuccguucgagauc" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "cacugauuccguucgagauc" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1339 " record
Length: 20
GC Content:50 %
Starting position: 23150
Strain of Virus: GZ50
GenBank Acc: AY304495
Transfection Reagent: Oligofectamine
Incubation Time (Hours):24

Offtargets for "cacugauuccguucgagauc" siRNA in Human Genome sequences
See AY304495 at Genbank
Pubmed:16638566
Article:Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.
Authors:He ML, Zheng BJ, Chen Y, Wong KL, Huang JD, Lin MC, Peng Y, Yuen KY, Sung JJ, Kung HF.
Journal:FEBS Lett. 2006 May 1;580(10):2414-20. Epub 2006 Mar 30.
Entrez:16638566

siRNA sequence "gggcuaccaccuuaugucc" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gggcuaccaccuuaugucc" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1287 " record
Length: 19
GC Content:58 %
Starting position: 3081
Strain of Virus: BJ01
GenBank Acc: AY278488
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gggcuaccaccuuaugucc" siRNA in Human Genome sequences
See AY278488 at Genbank
Pubmed:14988013
Article:Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference.
Authors:Zhang Y, Li T, Fu L, Yu C, Li Y, Xu X, Wang Y, Ning H, Zhang S, Chen W, Babiuk LA, Chang Z.
Journal:FEBS Lett. 2004 Feb 27;560(1-3):141-6.
Entrez:14988013

siRNA sequence "uguuguuauacgagcaugu" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "uguuguuauacgagcaugu" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1346 " record
Length: 19
GC Content:37 %
Starting position: 21857
Strain of Virus: Urbani
GenBank Acc: AY278741
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "uguuguuauacgagcaugu" siRNA in Human Genome sequences
See AY278741 at Genbank
Pubmed:15652970
Article:Inhibition of SARS-CoV replication by siRNA.
Authors:Wu CJ, Huang HW, Liu CY, Hong CF, Chan YL.
Journal:Antiviral Res. 2005 Jan;65(1):45-8.
Entrez:15652970

siRNA sequence "gacaaauagcgccaggaca" alignment with "SARS Coronavirus" virus reference Genome sequences
siRNA sequence matching with "gacaaauagcgccaggaca" SARS Coronavirus" Virus reference Genome sequences
Browse all the records for "SARS Coronavirus" virus
Browse "virsi1368 " record
Length: 19
GC Content:53 %
Starting position: 22660
Strain of Virus: CUHK-W1
GenBank Acc: AY278554
Transfection Reagent: Lipofectamine 2000
Incubation Time (Hours):48

Offtargets for "gacaaauagcgccaggaca" siRNA in Human Genome sequences
See AY278554 at Genbank
Pubmed:15848179
Article:Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Authors:Zhao P, Qin ZL, Ke JS, Lu Y, Liu M, Pan W, Zhao LJ, Cao J, Qi ZT.
Journal:FEBS Lett. 2005 Apr 25;579(11):2404-10.
Entrez:15848179


VIRsiRNAid	siRNA Sequence	Virus_Name	Target Region	Cell Line	Test Object	Efficacy	PMID	Offtarget	Align With	ALIGN0 Result
virsi1289	gggcuaccaccuuaugucc	SARS Coronavirus	Spike	Vero E6	mRNA	100	14988013	Blast SL	refseqs
virsi1294	gagcuuugagauugacaaa	SARS Coronavirus	Spike	293T	mRNA	91	15504339	Blast SL	refseqs
virsi1290	cccuuucuuugcuguuucu	SARS Coronavirus	Spike	293T	mRNA	90	15504339	Blast SL	refseqs
virsi1347	gggcuaucaaccuauagau	SARS Coronavirus	Spike	Vero E6	RNA	90	15652970	Blast SL	refseqs
virsi1288	gagacauaucuaaugugcc	SARS Coronavirus	Spike	Vero E6	mRNA	90	14988013	Blast SL	refseqs
virsi1291	gagacauaucuaaugugcc	SARS Coronavirus	Spike	293T	Protein	90	14988013	Blast SL	refseqs
virsi1344	caaggcgauuagucaaauu	SARS Coronavirus	Spike	Vero E6	RNA	85	15652970	Blast SL	refseqs
virsi1339	cacugauuccguucgagauc	SARS Coronavirus	Spike	FRhK-4	RNA	83	16638566	Blast SL	refseqs
virsi1287	gggcuaccaccuuaugucc	SARS Coronavirus	Spike	293T	Protein	80	14988013	Blast SL	refseqs
virsi1346	uguuguuauacgagcaugu	SARS Coronavirus	Spike	Vero E6	RNA	10	15652970	Blast SL	refseqs
virsi1368	gacaaauagcgccaggaca	SARS Coronavirus	Spike	293T	mRNA	3	15848179	Blast SL	refseqs



: siRNA specific for both strands
: siRNA specific for only one strands
: siRNA Not specific
SL: siRNA seedlocator algorithm